Nippon Genetics FastGene Scriptase III - 20000 Units at 200U/uL; 100 Reactions

The Scriptase III is an enhanced variant of the Murine Leukemia Virus (MuLV) reverse transcriptase. Due to specific mutations in the RNase H domain of the reverse transcriptase, cDNA can be synthesised even from very small amounts of RNA. Scriptase III has additional mutations that make it even more robust and thermostable. It can therefore operate with maximum activity up to a temperature of 55°C. At these elevated temperatures, highly structured RNAs can easily unfold, allowing them to serve as templates for reverse transcription. It is the superior choice for complex tasks, such as Real-Time PCR and NGS.

• An improved version of M-MLV Reverse Transcriptase, which exhibits high thermal stability (~55°C) and low RNase H activity
• Capable of synthesizing first strand cDNA at 42 ~ 55°C with high specificity and yield; at such high temperatures, extensively structured RNAs can be readily unfolded to serve as templates for reverse transcription
• SuperiorScript III can synthesise long cDNAs (up to ~12kb)
• Purity: ﹥99% on SDS-PAGE
• Endonuclease- and exonuclease-free
• RNase-free
• Inhibitor-free
• Satisfactory cDNA product yield and length
• Comes with additional components that are needed for reverse transcription, it consists of 5 vials including:
• FastGene® Scriptase III Restriction Enzyme
• 5x First-Strand Buffer (250mM Tris-HCl (pH 8.3), 375mM KCl, 15mM MgCl₂)
• 0.1M DTT
• dNTP mix (10mM each)
• Sterile water (RNase free)